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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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Ac-DEVD-AMC is a fluorescent substrate designed for caspase-3 and caspase-7. When incubated with cell lysate, it releases amino-4-methylcoumarin (AMC), enabling fluorescence detection at specific wavelengths.
Fluorescent substrate for caspase-3/caspase-7
Releases amino-4-methylcoumarin (AMC) for fluorescence detection
Excitation wavelength of 380 nm
Emission wavelength of 460 nm
For research use only
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L uciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2 as cofactors to emit a characteristic yellow-green emission in the presence of oxygen which shifts to red light in vivo at 37 C Through the utilization of ATP the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain
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VivoGenie Anti-Human HER2 (Pertuzumab) Biosimilar is a vivogenie with human reactivity Available in 1mg size with 7 - 14 business days lead time Ships via gel pack Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month For longer term storage aseptically aliquot in working volumes without diluting and store at -80 C Avoid Repeated Freeze Thaw Cycles
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A fluorometric FAAH substrate used for enzyme activity measurement; release of the fluorescent AMC (ex/em = 360/465 nm) allows for fast and convenient measurement of FAAH activity
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MW 535.6 Da, Purity >98%. Yellow chromogenic β-lactamase substrate. Hydrolyses readily in the presence of all β-lactamases except for Aeromonas hydrophila metalloenzyme. Suitable for kinetic studies, the detection of the enzymes in crude extracts and chromatographic fractions. Absorbs light at 346 nm (Δε = -2500 M-¹ cm-¹) or 405 nm (Δε = +6400 M-¹ cm-¹).
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A fluorogenic peptide substrate; used to assess the activity of bacterial proteases from P. aeruginosa and S. aureus; enzyme activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm
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Coelenterazine hcp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 190 times higher and has a faster response time to calcium compared to native coelenterazine.Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator. It binds coelenterazine to form an aequorin complex that emits light upon calcium binding and measures concentrations ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter of transcription regulation and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines are also used for chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues. Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines are also available.Properties : Yellow solid soluble in MeOH or EtOH; Store at -20°C, protect from light; C25H25N3O2; MW: 399.49.
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Available as a substrate component of the HistoMark Orange immunohistochemistry substrate kit or as a stand-alone product. Requires Enhance Orange Buffer and Peroxide Solution for use.
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D-Luciferin is a popular bioluminescent substrate of luciferase in the presence of ATP used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications In an immunocompetent mouse model of ovarian cancer the use of D-luciferin substrate and firefly luciferase preserves tumour-host immune interactions since bioluminescent imaging is a more sensitive indication of tumour growth than weight gain
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Irreversible, cell permeable inhibitor of caspase-1. Also inhibits caspase-4. Alternative name: Caspase 1 inhibitor, Ac-Tyr-Val-Ala-Asp-2,6-dimethylbenzoyloxymethylketone. Formula: C33H42N4O10. MW: 654.7. Purity: ≥95% (HPLC). Appearance: White powder. Peptide content: 78-99%. Solubility: Soluble in DMSO or methanol. Long Term Storage: -20°C. Use/Stability: Stock solutions should be divided into aliquots and stored at -20°C. Handling: Keep cool and dry.
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